Product Overview

Water HPLC Gradient Grade (CAS No. 7732-18-5) is high-purity chromatographic water intended particularly for gradient HPLC, where changes in mobile-phase composition can reveal accumulated trace contaminants as baseline drift, ghost peaks or rising detector background. Gradient methods can concentrate weakly retained impurities on the column before they elute as the organic-solvent proportion increases, making solvent cleanliness especially important.

The HPLC Gradient Grade is intended particularly for gradient mobile-phase preparation, sensitive chromatographic blanks, sample dilution and method development. Its grade relevance centres on maintaining a clean aqueous background as mobile-phase composition changes during gradient elution.

Typical Applications

• Aqueous component of gradient HPLC mobile phases.
• Gradient blank and ghost-peak investigations.
• Preparation of HPLC gradient buffers.
• Sample dilution for gradient chromatographic methods.
• Baseline-sensitive HPLC method development.
• Column-cleanliness and mobile-phase background studies.

Common Synonyms

• Aqua
• Dihydrogen monoxide
• Dihydrogen oxide
• Oxidane

Storage Conditions

Keep tightly closed and protect rigorously from organic, particulate, ionic and microbiological contamination. Use dedicated clean dispensing systems to minimise introduction of trace contaminants that could accumulate during gradient chromatography.