Product Overview

EDTA Disodium Salt Dihydrate 99% For Molecular Biology (CAS No. 6381-92-6) is the dihydrated disodium salt of ethylenediaminetetraacetic acid, a polyaminocarboxylic acid and multidentate chelating agent. Its four carboxylate groups and two nitrogen donor centres enable strong complex formation with numerous metal ions, particularly divalent cations such as calcium and magnesium. This metal-binding behaviour is central to the use of disodium EDTA in biochemical systems, where sequestration of available metal ions can alter metal-dependent reactions and enzymatic activity.

The Molecular Biology grade is intended for preparation of biological buffers and reagent systems used in nucleic-acid and biomolecular procedures. EDTA is routinely incorporated into formulations such as nucleic-acid extraction buffers and TE-type systems, where chelation of divalent cations helps limit metal-dependent nuclease activity and contributes to preservation of DNA and RNA during handling. It is also relevant to electrophoresis, cell lysis, enzyme studies and other molecular-biology workflows in which controlled availability of metal ions is required.

Typical Applications

• Preparation of TE and related EDTA-containing molecular-biology buffer systems.
• DNA extraction, isolation and purification procedures.
• RNA extraction and purification workflows.
• Preparation of nucleic-acid lysis and storage buffers.
• Electrophoresis buffer and reagent preparation where EDTA chelation is appropriate.
• Molecular-biology procedures requiring sequestration of divalent metal ions to control metal-dependent enzymatic activity.
• Preparation of biochemical and cell-biological reagent systems requiring a chelating agent.

Common Synonyms

• Ethylenediaminetetraacetic Acid Disodium Salt Dihydrate
• Disodium Ethylenediaminetetraacetate Dihydrate
• Disodium EDTA Dihydrate
• Edetate Disodium Dihydrate
• EDTA Disodium Salt
• EDTA-Na₂
• Edetate Disodium
• Sodium Versenate

Storage Conditions

Store tightly closed in a dry, clean environment and protect the material from unnecessary exposure to atmospheric moisture, contamination and excessive heat during storage and handling. Promptly reseal the container after dispensing to maintain the intended dihydrate form and preserve suitability for preparation of molecular-biology buffers and reagent systems.